WebAlthough cyclin-dependent kinase 2 (CDK2) is a validated target for both cancer and contraception, developing a CDK2 inhibitor with exquisite selectivity has been … WebAug 19, 2011 · The assay is based on the specific interaction of CDK2 with the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS), which binds to a large allosteric pocket adjacent to the ATP site. Hit compounds that displace ANS directly or indirectly from CDK2 are readily classified as ATP site binders or allosteric ligands through the use of ...
Cooperativity Between Orthosteric Inhibitors and Allosteric …
WebOct 1, 2015 · Significantly, B2 certainly interrupted the interaction between CDK2 and cyclin A3 and exhibited a concentration-dependent trend. In summary, our results suggest that B2 is the first effective allosteric chemical small-molecule CDK2 inhibitor to be discovered, and further lead optimization may result in a series of novel anti-CDK2 agents. Weballosteric pocket with higher affinity and with better drug-like properties is highly desirable. In this work we have performed a virtual screening of commercially available compounds (Asinex collection, ~600000 compounds) in the allosteric pocket of the CDK2–ANS binary complex. The screening was conducted by using a combination literature for composition 11th edition
Allosteric Modulators of Protein–Protein Interactions (PPIs)
WebAllosteric targeting of protein kinases via displacement of the structural αC helix with type III allosteric inhibitors is currently gaining a foothold in drug discovery. Recently, the first crystal structure of CDK2 with an open allosteric pocket adjacent to the αC helix has been described, prospec … WebJan 10, 2024 · The inside cover picture shows the first small-molecule allosteric inhibitor of cyclin-dependent kinase 2 (CDK2) binding within an allosteric pocket in the proximity … WebJan 30, 2024 · Additionally, EF-4-117 bound much more strongly to the CDK2 pocket than the dye, making it the highest affinity inhibitor for this site reported to date. ... Allosteric Cyclin-Dependent Kinase 2 ... literature for children\u0027s church