WebSep 9, 2024 · HDF (Lonza, CC-2509) were cultured in filter-sterilized (0.22 μm, Corning) DMEM (Corning 10013CV) supplemented with 10% FBS (ATCC SCRR-30-2024TM) and 1% penicillin-streptomycin (P/S, Sigma-Aldrich) under standard culturing condition (37°C, humidified, 5% CO 2 /95% air). Cells at passage number of 4–6 were used in the … WebMar 22, 2024 · Enzyme digestion was inhibited with inhibition media containing DMEM (Corning; #10013CV), 20% FBS, 1 m m EDTA (UltraPure 0.5 m EDTA, pH 8.0; Thermo Fisher Scientific; #15575-038) and P/S. The tissue solution was filtered (70 μm and 40 μm) to obtain mononuclear cell suspension, and the filtrate was centrifuged at 300 RCF for 5 …
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WebPrice from $9.99 to $1999.99 adsc growth medium - by Bioz Stars , 2024-04 96 / 100 stars Images 1) Product Images from "Human beige adipocytes for drug discovery and cell … WebMar 3, 2024 · Briefly, spheroids were generated by seeding approximately 1 × 10 3 cells in each of the 96 wells of an ultra-low attachment plate (Corning, No. 7007) and allowed to form for 48 h in the presence ... g0176 and h2032
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WebMT10013CV Corning™ DMEM with L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original … WebApr 1, 2024 · Cos-7 cells (ATCC, CRL-1651) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Corning, 10013CV) containing L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine ... WebJun 2, 2024 · To passage cells from a 100 mm cell culture plate, ADSCs were washed with DPBS (Corning, 21031CV) and then incubated with 5 ml of Accutase (Innovative Cell … glass coffee percolator vintage