WebFlow cytometric analysis of adipocytes from wild type mice, untransfected (A) or transfected with Poly (I:C) (2.5 µg/ml, 6 hr; B), and adipocytes from IRF-3 (-/-) mice, untransfected (C) or transfected with Poly (I:C) (2.5 µg/ml, 6 hr; D), using Phospho-IRF … WebJan 18, 2024 · The TFs interferon regulatory factor 3 (IRF3), IRF5 and IRF7 (IRF3/5/7) ... To understand the role of DNA binding in IFN regulation, we mapped the binding of IRF3/5/7 dimers to all human and mouse IFN gene VREs. We found that IRF3 and IRF7 bind across many of the VRE sub-elements, but that the VRE-A/B sub-element is bound almost …
Phospho-IRF-3 (Ser396) Antibody Cell Signaling Technology
WebJul 7, 2024 · However, the published Irf3 −/− mice that were previously tested are also deficient in Bcl2l12, a gene that neighbors Irf3 and which was inadvertently disrupted by the deletion targeting Irf3 44. WebIn this study, we analyzed the role of IRF3 and IRF7 for the host response to IAV infections in Irf3-/-, Irf7-/-, and Irf3-/-Irf7-/- knockout mice. While the absence of IRF3 had only a … sharon barbour psac
IRF3-binding lncRNA-ISIR strengthens interferon production
WebMar 26, 2015 · Interferon Regulatory Factor 3 (IRF3) is an essential transcription factor for the expression of antiviral genes, including type I IFNs and ISGs. ... Ser398 in human IRF3 and Ser388, Ser390 in mouse IRF3) . The known signaling pathways activate IRF3 through its phosphorylation by the protein kinase, TBK1, which in turn is activated by signal ... WebFor our assay, we are attempting to detect IRF3 activation via dimerization in mouse macrophage nuclear and cytoplasmic lysates run on a native gel. It is important for us … WebThe IRF3 conditional knockout mouse may be a useful tool to identify cell type-specific functions of this transcription factor. Abstract IFN regulatory factor 3 (IRF3) is a … population of sarasota florida 2021