Thymidine uptake

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WebbThymidine uptake was assayed in L1210 cells using a modifica-tion of the rapid mixing technique of Wohlheuter et al (1978). The uptake of 100 mM thymidine by 106 cells was followed at 2-second WebbTymidinkinas krävs för verkan av många antivirala läkemedel. Det används för att välja hybridomcellinjer för produktion av monoklonala antikroppar. I klinisk kemi används det som en markör vid diagnos, kontroll av behandling och uppföljning av maligna sjukdomar, främst av hematologiska maligniteter . Innehåll 1 Historia 2 Klassificering 3 Biokemi

Thymidine uptake in vitro as a prognostic indicator for ... - PubMed

WebbThe dose and time effects of different concentrations of corrosion products on the growth and morphology of smooth muscle cells were evaluated with [3H]-thymidine uptake ratio and cell cycle sorter. Both the supernatant and precipitate of the corrosive products of nitinol wire were toxic to the primary cultured rat aortic smooth muscle cells. WebbThymidine Incorporation Assays We are now two standalone organizations Life Sciences & Diagnostics A Life Sciences and Diagnostics business focused on enabling scientists … factory 300 blackout bolt guns

Elevation of radiolabelled thymidine uptake in RIF-1 ... - PubMed

Webb[(3)H]thymidine uptake decreased at 24 and 48 h post treatment. AG337 also produced a similar effect. In contrast to the TS inhibitors, cisplatin decreased [(3)H]thymidine … Webb1 apr. 1977 · The initial concentration of thymidine in the growth medium was 0.35 microng/ml while the initial concentration of purine nucleosides ranged from 25 to 250 … WebbWe have investigated the relationship between cell numbers and the amount of tritiated thymidine ([3H]TdR) taken up by stimulated human peripheral lymphocytes, as a … factory319

Quantitation of [3H] thymidine uptake by stimulated human

Renal enlargement: comparative autoradiographic studies of 3H …

WebbThymidine uptake into the acid-soluble fraction of several hamster cell lines differing in levels of thymidine kinase was examined. Uptake is correlated directly with the level of this enzyme, a kinase-deficient line showing the lowest uptake rate. At low extracellular concentrations the major portion of the uptake that occurs is related to phosphorylation, … Webb19 juli 2013 · With the idea of finding a more selective radiotracer for imaging herpes simplex virus type 1 thymidine kinase (HSV1-tk) gene expression by means of positron emission tomography (PET), a novel [18F]fluorine radiolabeled pyrimidine with 4-hydroxy-3-(hydroxymethyl)butyl side chain at N-1 (HHB-5-[18F]FEP) was prepared and evaluated as … factory 30Webb6 maj 2024 · With exposure of the GES-1 cells to 64 μg/mL quercetin, no loss of cell viability was observed after 48-h culture ( p < 0.05) ( Figure 4 A). [ 3 H] thymidine uptake is a simple way to measure cellular proliferation [ 28 ]. If cellular proliferation is stopped, the drug toxicity is often considered. factory 305

"WebbSince thymidine is one of the key components of DNA, measuring incorporation of the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) into DNA provides a reflection of the rate of DNA synthesis. Cells are plated and quiesced in serum-free media as above. Then, cells are stimulated with vehicle or a mitogen, such as 10% FBS. " - Thymidine uptake

Thymidine uptake

Increased Uptake of Thymidine in - JSTOR

WebbUptake of tritiated thymidine is assessed by liquid scintillation counting of samples blotted onto filter discs ( Knight, 1987 ). More recently, instead of measuring counts in … WebbThymidine Uptake, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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WebbCellular uptake of [3H]thymidine [ ( 3H]TdR) and incorporation into DNA of Ehrlich ascites tumour cells were studied in relation to the cell cycle by measuring the activity in the … Webb6 sep. 2024 · In this review, we describe the potential therapeutic usages of CPPs (mainly trans-activator of transcription protein, TAT) in enabling the uptake of biologically active proteins/enzymes needed in cases of protein/enzyme deficiency, concentrating on mitochondrial diseases and on the import of enzymes or peptides in order to destroy …

Webb1 dec. 1977 · Significantly increased thymidine labeling was found only in patients with the most severe changes of papillary length or basal layer thickening. We therefore conclude that there is an increased basal zone uptake of [3 H]thymidine in severe esophagitis which suggests increased cell turnover as the mechanism for basal zone hyperplasia. WebbFirst thymidine block. Add thymidine to the cells to a final concentration of 2 m M and incubate for 8 h. 3. Wash the cells three times with 1 × PBS. 4. Add complete DMEM for 4 …

WebbAssay Description. The thymidine incorporation assay, the most common assay, utilizes a strategy wherein a radioactive nucleoside, 3H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division. A scintillation beta-counter is used to … Webbthymidine uptake. Compared to phytohemagglutinin (PHA; 5 μg/mL) stimulation, 2-OH significantly enhanced PBMC proliferation in a dose-dependent manner. The 50% enhancement activity (EC50) for 2-OH was 4.4±0.1 μM. In addition, effects of 2-OH on interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in PBMC were determined by …

Webbthymidine uptake in fertilized eggs, the binding through a 45 mm comparison, cumulative uptake of [3H]-thymidine was Apparently, binding of NBMPR to sur measured at 60 min post-insemination in ^ace on fertilized sea urchin eggs is the presence of increasing concentrations not Na+-dependent, of NBMPR. Figure 2 shows the reduc

Webb15 nov. 1993 · Background: Prognostic significance of in vitro thymidine uptake by cancer cells remains unclear in patients with gastric cancer. Methods: In 173 patients with … does tourism ruin the citiesWebb11 apr. 2024 · Cellular uptake of a fluorescent negative control PPRH (NC) in MDA-MB-453 cells (330,000 cells/well) was analyzed by fluorescent microscopy (a) and flow cytometry (b), 24 h after transfection with either 300 or 400 nM of the PPRH labeled with fluorescein (FAM) and 30 or 40 µM of DOTAP, respectively. does towel on forehead feverWebbThe thymidine incorporation assay, the most common assay, utilizes a strategy wherein a radioactive nucleoside, 3H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division. factory 302 pushrod lengthWebb14 jan. 2024 · When the level of 14 C-thymidine uptake for each group was measured as a percentage by LSC, it revealed a decline in uptake efficiency for AD-MSCs that received concentrations of 10 and 25... factory 31Webb31 okt. 2009 · Immune-deficient PNP-/- mice had increased [(3)H]thymidine and EdU incorporation into non-stimulated spleen cells, indicative of spontaneous proliferation. … does toward have an sWebbThymidine uptake and utilization in Escherichia coli: a new gene controlling nucleoside transport A commonly used strain of Escherichia coli K-12 was shown to be deficient in … does tourism hurt hawaiiWebbHeterotrophic marine bacterium isolates from Tampa Bay, Fla., Chesapeake Bay, Md., and a coral surface microlayer were examined for thymidine uptake (transport), thymidine incorporation, the presence of thymidine kinase genes, and thymidine kinase enzyme activity. Of the 41 isolates tested, 37 were capable of thymidine incorporation into DNA. factory 319